Nucleic Acid Extraction lossis Purification Kit
Nucleic Acid Extraction Or Kev huv huv Kitlos yog khaws cia ntawm -20 ℃. Cov qauv yuav tsum raug thauj siv 0 ℃ curling.
Introduction
Nucleic Acid Extraction lossis Purification Kit (Magnetic Beads Method) yog tsim los rau kev ua kom huv huv ntawm RNA thiab DNA los ntawm cov kua hauv lub cev (xws li swabs, plasma, serum) siv cov cuab yeej siv hluav taws xob nucleic acid extraction. Magnetic-particle technology muab cov khoom zoo DNA / RNA uas tsim nyog rau kev siv ncaj qha rau hauv cov ntawv sau cia xws li kev nthuav dav lossis lwm yam tshuaj tiv thaiv enzymatic.
Application Range
Tag nrho cov ntshav, ntshav, ntshav ntshav thiab lwm yam ntaub so ntswg kuaj tau ncaj qha lysed thiab digested. Lub tso tawm nucleic acid tau xaiv adsorbed los ntawm super paramagnetic nanometer magnetic hlaws. Tom qab ntawd cov protein, inorganic ntsev ions thiab cov organic impurities raug tshem tawm los ntawm kev ntxuav cov kua. Thaum kawg, cov nucleic acid tau eluted nrog eluent kom tau ib tug ntshiab nucleic acid tov.
Kit Contents
| Cat. Tsis muaj. | YXN-VIRAL01-32A-BR | Cheebtsam | ||
| -50 A | - 100 A | |||
| Loj | 32 Tes | 50 Test | 100 Test | |
| Lysis Buffer | 96 wellPre-pack ed Daim phiaj 2 qab | 25ml ib | 50 ml | Surfactant thiab Tris |
| Ntxuav Buffer I | ★ 15 ml | ★ 30ml | Cov kua ntsev ntau | |
| Ntxuav Buffer Il | 6ml * 2 | 12ml * 2 | Tsawg ntsev tov | |
| Elution Buffer | 10 ml | 20 ml | Tsawg ntsev tov | |
| MagaBio Reagents | 1.0ml ib | 2.0ml ib | Sib nqus particle | |
| Phau ntawv (=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Notes:RauYXN-VIRAL01-32A-BR-50A,ntxiv 15mL Absolute ethanol rau ★15mL Ntxuav Buffer Kuv ua ntej siv; Ntxiv 24mL Absolute ethanol rau ★6mL Ntxuav buffer Il ua ntej siv. | ||||
| RauYXN-VIRAL01-32A-BR-100A, ntxiv 30mL Absolute ethanol rau ★30mL Ntxuav Buffer Kuv ua ntej siv; Ntxiv 48mL Absolute ethanol rau ★12mL Ntxuav buffer Il ua ntej siv. 【Reagents yuav tsum tau npaj los ntawm tus neeg siv】Thov npaj lub meej ethanol (kib tsom xam) ntawm koj tus kheej. | ||||
Stlus Condnwsons
Thaum tuaj txog ntawm cov khoom siv, cov khoom siv tuaj yeem khaws cia ntawm chav tsev kub (15 - 25 ° C). Cov reagents ruaj khov mus txog ib xyoos txij li hnub tsim khoom.
Sample Requirements
1. Cov qauv siv tau: Swabs, plasma, serum thiab tag nrho cov ntshav thiab lwm yam.
2. Cov qauv khaws cia thiab kev thauj mus los: Cov qauv yuav tsum tau kuaj tam sim
Materials thiab Devices Required but Not Provided
1. Cov hnab looj tes tsis muaj hmoov pov tseg
2. Biohazard thawv
3. cwj mem los yog ib
Procegre
Cov hauv qab no siv cov qauv strip extraction swab lotion ua piv txwv los piav qhia luv luv txog kev ua haujlwm ntawm cov extraction reagent ntawm cov khoom siv roj ntsha nucleic acid extraction.Bioer NPA-32p iblos yogSMART 32. Rau lwm hom qauv, thov xa mus rau tus neeg siv phau ntawv. Nws tseem tuaj yeem ua haujlwm los ntawm cov neeg siv khoom raws li kev sim tau txais:
1. Reagent Kev npaj
ib. RauYXN-VB03-32A-50A thiab YXN-VB03-32A-100A
Ntxiv 500uL Lysis Buffer rau kem 1 thiab 7 ntawm 2.2mL 96-dub-zoo phaj, 500uL Ntxuav Buffer I mus rau sab 2 thiab 8, 500uL Wash Buffer II mus rau sab 3, 4 thiab 9,10; 70uL Elution Buffer rau kem 5 thiab 11, 180uL cov dej ntshiab thiab 20uL MagaBio Reagent rau sab 6 thiab 12 (cov hlaws sib nqus yuav tsum tau muab sib xyaw ua ntej siv),
b. RauYXN-VB03-32A
Muab 96 zoo pre-packed reagents nyob rau hauv chav tsev kub. Co 96-zoo phaj upside down rau peb zaug, thiab rhuav tshem lub hnab yas. Centrifuge lub pre-packed reagent rau ob peb feeb (los yog viav vias ntawm tes ob peb zaug) kom tsis txhob reagent adhering rau phab ntsa ntawm cov hlab. Tshem tawm cov ntawv ci aluminium ntawm 96-zoo phaj thiab txheeb xyuas cov kev taw qhia ntawm lub phaj (sib nqus hlaws hauv kab # 6 thiab # 12),
2.Qauv Kev rho tawm
1. Ntxiv 300uL qauv rau 96 qhov zoo phaj kab #1 thiab #7, thov kom tsis txhob kis kab mob sib kis,
2. Muab 96 qhov sib sib zog nqus phaj rau lub ntsuas, nruab 8-kab lus qhia ntawm lub ntsuas,
3. Khiav qhov kev pab cuam raws li cov txheej txheem hauv qab no,
4. Tom qab tsis siv neeg purification dhau lawm, hloov lub elution Buffer nyob rau hauv kab 5 thiab 11 mus rau ib tug huv si antinuclear 0.5mL centrifuge raj; Yog tias tsis siv tam sim ntawd, thov khaws cia ntawm -20 ° C.
Performance Characteristics
1. Cov khoom muab rho tawm yog kuaj pom los ntawm high-rhiab heev HBV DNA kuaj reagent kom ncav cuag rhiab heev ntawm 10 IU/mL. Cov khoom rho tawm tau raug kuaj pom los ntawm qhov siab-rhiab heev HCV RNA tshawb pom reagent kom ncav cuag qhov rhiab heev ntawm 50 IU / mL.
2. Xaiv 4 cov qauv (cov qauv ntshav / ntshav, cov qauv nasopharyngeal swab, cov qauv ntawm lub ncauj tsev menyuam exfoliated cell), txhua tus qauv yog diluted 10 zaug nrog 3 gradients (suav nrog cov qauv qub tag nrho ntawm 4 concentrations), siv cov tshuaj reagents thiab cov neeg ua haujlwm kuaj xyuas kom paub txog sab hauv. siv cov noob raws li cov lus qhia khoom, thiab tus nqi Ct ntawm txhua pawg sib txawv los ntawm tsawg dua 1.
| Step | Zoo Qhov chaw | Program Lub npe | Wanwsng Time(min:SS) | Mixhauv Time(mhauv:SS) | Magnet Time(min:SS) | Adsorption | Speb | Volum Taus (μL) | Thuab tais |
| 1 | 1 | Lysis | 0:0 ua0 | 2:00 | 0:0 ua0 | F | 700 | 80 | |
| 2 | 6 | Bua eds | 0:0 ua0 | 0:15 ua | 0:15 ua | √ | F | 200 | |
| 3 | 1 | Bind | 0:0 ua0 | 3:0 ua0 | 0:45 ua | √ | F | 700 | |
| 4 | 2 | Wash1 ib | 0:0 ua0 | 0 :30 | 0 :30 | √ | F | 500 | |
| 5 | 3 | Wua ash2 | 0:0 ua0 | 0 :30 | 0 :30 | √ | F | 500 | |
| 6 | 4 | Wash 3 | 0:0 ua0 | 0 :30 | 0 :30 | √ | F | 500 | |
| 7 | 5 | Elution | 2:00 | 2:30 | 0 :30 | F | 70 | 80 | |
| 8 | 6 | Muab pov tseg | 0:0 ua0 | 0:15 ua | 0:0 ua0 | F | 200 |
Safety
1. GENERAL SAIB.
Kev siv cov khoom no raws li qhov tsis tau teev tseg hauv cov ntaub ntawv tus neeg siv yuav ua rau tus kheej raug mob lossis kev puas tsuaj rau lub cuab yeej lossis khoom siv. Xyuas kom meej tias ib tus neeg siv cov khoom no tau txais cov lus qhia hauv kev coj ua kev nyab xeeb dav dav rau cov chaw sim thiab cov ntaub ntawv kev nyab xeeb muaj nyob hauv daim ntawv no.
1.1 Ua ntej siv lub cuab yeej lossis cov cuab yeej, nyeem thiab nkag siab txog cov ntaub ntawv kev nyab xeeb uas tau muab hauv cov ntaub ntawv siv los ntawm cov chaw tsim khoom ntawm cov cuab yeej lossis cov cuab yeej.
1.2 Ua ntej tuav cov tshuaj, nyeem thiab nkag siab tag nrho cov ntaub ntawv Kev Nyab Xeeb (SDSs) thiab siv cov cuab yeej tiv thaiv tus kheej tsim nyog ( hnab looj tes, ris tsho, tiv thaiv qhov muag, thiab lwm yam). Yuav kom tau txais SDSs, saib "Cov Ntaub Ntawv thiab Kev Txhawb" nyob rau hauv daim ntawv no.
2. Tshuaj kev nyab xeeb
GENERAL CHEMICAL HLOOV. Txhawm rau txo qhov phom sij, xyuas kom cov neeg ua haujlwm hauv chav kuaj nyeem thiab ua raws li cov lus qhia txog kev nyab xeeb rau kev siv tshuaj lom neeg, kev khaws cia, thiab pov tseg hauv qab no, thiab sab laj nrog SDS muaj feem xyuam rau kev ceev faj thiab cov lus qhia tshwj xeeb: Nyeem thiab nkag siab txog Daim Ntawv Qhia Kev Nyab Xeeb (SDSs) muab los ntawm cov tshuaj. chaw tsim tshuaj paus ua ntej koj khaws, tuav, los yog ua hauj lwm nrog tej yam tshuaj los yog tej yam khoom uas txaus ntshai..
2.1 Txo kev sib cuag nrog tshuaj. Hnav cov khoom tiv thaiv tus kheej tsim nyog thaum tuav cov tshuaj (piv txwv li, tsom iav, hnab looj tes, lossis khaub ncaws tiv thaiv).
2.2 Txo qhov nqus ntawm cov tshuaj. Tsis txhob qhib cov thawv ntim tshuaj. Tsuas yog siv qhov cua kom txaus (piv txwv li, fume hood).
2.3 Tshawb xyuas tsis tu ncua rau cov tshuaj xau los yog nchuav. Yog tias muaj dej los yog nchuav, ua raws li cov chaw tsim khoom cov txheej txheem ntxuav raws li pom zoo hauv SDS.
2.4 Tuav cov khoom pov tseg hauv lub fume hood.
2.5 Xyuas kom siv cov khoom pov tseg thawj zaug thiab theem nrab. (Ib qho khoom pov tseg
thawv tuav cov pov tseg tam sim ntawd. Ib lub thawv thib ob muaj cov nchuav los yog xau los ntawm lub thawv thawj. Ob lub thawv yuav tsum sib haum nrog cov khoom pov tseg thiab ua tau raws li tsoomfwv, lub xeev, thiab cov kev cai hauv zos rau cov thawv ntim khoom.).
2.6 Tom qab muab lub thawv pov tseg, muab khi nrog lub kaus mom.
2.7 Tus cwj pwm (los ntawm kev txheeb xyuas yog tias tsim nyog) cov khoom pov tseg uas tsim los ntawm cov ntawv thov tshwj xeeb, cov tshuaj reagents, thiab cov khoom siv hauv koj chav kuaj.
2.8 Xyuas kom meej tias cov khoom pov tseg tau khaws cia, xa mus, thauj, thiab pov tseg raws li tag nrho cov cai hauv zos, xeev / xeev, thiab / lossis lub tebchaws.
2.9 Cov khoom siv hluav taws xob lossis cov khoom siv biohazardous yuav tsum tau tuav tshwj xeeb, thiab kev txwv kev pov tseg yuav siv tau.
3.Kev lom neeg phom sij kev nyab xeeb
Muaj peev xwm Biohazard. Nyob ntawm cov qauv siv ntawm qhov ntsuas no, qhov saum npoo yuav raug suav hais tias yog biohazard. Siv cov txheej txheem decontamination tsim nyog thaum ua haujlwm nrog biohazars.
BIOHAZARD. Cov qauv tshuaj lom neeg xws li cov ntaub so ntswg, cov kua dej hauv lub cev, cov kab mob sib kis, thiab cov ntshav ntawm tib neeg thiab lwm yam tsiaj muaj peev xwm kis tau tus kab mob. Ua raws li tag nrho cov kev cai hauv zos, xeev/xeev, thiab/lossis lub tebchaws. Hnav cov cuab yeej tiv thaiv tsim nyog, uas suav nrog tab sis tsis txwv rau: cov looj tsom iav tiv thaiv, daim npog ntsej muag, khaub ncaws / hnab looj tes, thiab hnab looj tes. Txhua txoj haujlwm yuav tsum tau ua nyob rau hauv cov chaw tsim khoom kom zoo siv cov cuab yeej kev nyab xeeb tsim nyog (piv txwv li, cov cuab yeej tiv thaiv lub cev). Cov tib neeg yuav tsum tau txais kev cob qhia raws li kev tswj hwm thiab lub tuam txhab / tsev kawm ntawv cov cai ua ntej ua haujlwm nrog cov ntaub ntawv uas muaj feem kis tau.
Nyeem thiab ua raws li cov lus qhia siv tau thiab / lossis cov cai tswj hwm hauv cov hauv qab no:
Hauv Teb Chaws Asmeskas: Lub Tsev Haujlwm Saib Xyuas Kev Noj Qab Haus Huv thiab Tib Neeg Txoj Cai tau tshaj tawm hauv Biosafety hauv Microbiological thiab Biomedical Laboratories pom ntawm: www.cdc.gov/biosafety.
Kev Nyab Xeeb Kev Nyab Xeeb thiab Kev Noj Qab Haus Huv Standards, Bloodborne Pathogens (29 CFR§1910.1030), pom ntawm:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Koj lub tuam txhab / lub koom haum Biosafety Program raws tu qauv rau kev ua hauj lwm nrog / tuav cov ntaub ntawv uas yuav kis tau. Cov ntaub ntawv ntxiv txog cov txheej txheem biohazard muaj nyob ntawm: www.cdc.gov.
Hauv EU: Tshawb xyuas cov lus qhia hauv zos thiab kev cai lij choj ntawm biohazard thiab biosafety ceev faj thiab xa mus rau cov kev coj ua zoo tshaj plaws uas tau tshaj tawm hauv World Health Organization (WHO) Laboratory Biosafety Manual, thib peb tsab, pom ntawm: www.who.int/csr/resources/publications /biosafety/WHO_CDS_CSR_LYO_200 4_11/en/.
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